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Morpholino oligos and transfection of morpholino worldd in regenerating limbs Fluorescein-conjugated morpholino oligos were purchased from Gene Tools.
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C RT-PCR analysis of PL1 and PL2 transcriptional activity in the intact limb epidermis or mesenchyme, and in a healing superficial limb skin wound and regenerating limb blastemal epidermis and mesenchyme. Deep blue dots were added along the outlines of regenerating limbs to make the regenerating limbs more visible.
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